dox诱导的shRNA敲低实验中如何设置对照组
在生物医学研究中，RNA干扰（RNAi）技术是探索基因功能的重要工具。基于四环素调控系统（Tet-On/Tet-Off）的dox（多西环素）诱导型shRNA表达体系，因其可时空特异性调控基因表达而备受青睐。然而，这类实验的结论可靠性高度依赖于对照组的合理设计。本文将对dox诱导实验中对照组的科学逻辑进行解析。
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Differences Between U6, EF1α, and Ubc Promoters
The U6 promoter originates from the RNA polymerase III (Pol III) system and is used to drive the expression of non-coding RNAs. Its most common applications include driving the expression of small RNA molecules such as short hairpin RNA (shRNA) and small guide RNA (sgRNA). If you need to express short RNA molecules like shRNA or sgRNA for RNA interference (RNAi) or CRISPR gene editing, the U6 promoter is the best choice. For efficiently and stably expressing exogenous proteins in a variety of cell lines, the EF1α promoter is a powerful and durable option, especially suited for long-term expression in lentiviral vector systems. The Ubc promoter is ideal for applications requiring stable gene expression with moderate strength, particularly in experiments where immunogenicity is a concern, such as with stem cells or primary cells.